Data from minimal gene set whole-cell modelling

The minimal gene set for life has often been theorised, with at least ten produced for Mycoplasma genitalium (M. genitalium). Due to the difficulty of using M. genitalium in the lab, combined with its long replication time of 12 - 15 hours, none of these theoretical minimal genomes have been tested, even with modern techniques. The publication of the M. genitalium whole-cell model provided the first opportunity to test them, simulating the genome edits in-silico. We simulated minimal gene sets from the literature, finding that they produced in-silico cells that did not divide. Using knowledge from previous research, we reintroduced specific essential and low essential genes in-silico; enabling cellular division. This reinforces the need to identify species-specific low essential genes and their interactions. Any genome designs created using the currently incomplete and fragmented gene essentiality information, will very likely require in-vivo reintroductions to correct issues and produce dividing cells.

Alternative title Testing theoretical minimal genomes using whole-cell models
Creator(s) Lucia Marucci, Claire Grierson, Oliver Chalkley, Joshua Rees, Jake Rightmyer
Publication date 04 May 2021
Language eng
Publisher University of Bristol
Licence Non-Commercial Government Licence for public sector information
DOI 10.5523/bris.356boayld729v2snyncfoltt0w
Complete download (zip) https://data.bris.ac.uk/datasets/tar/356boayld729v2snyncfoltt0w.zip
Citation Lucia Marucci, Claire Grierson, Oliver Chalkley, Joshua Rees, Jake Rightmyer (2021): Data from minimal gene set whole-cell modelling. https://doi.org/10.5523/bris.356boayld729v2snyncfoltt0w
Total size 261.9 GiB

Data Resources