Image analysis of apoptopic hallmarks

The macros provided are designed to analyse the progression of apoptosis.

"nuclei analysis_NAF calculation_single stack" focuses on providing nuclei area measurements and calculating the nuclear area factor (NAF). This is to be performed on single stack images.

"Caspase 9_NAF activity analysis", in addition to calculating the NAF, caspase 9 activity is also quantified. This macro is to be performed on Z-stack images.

These macros were designed and tested upon Chinese hamster ovary (CHO)-K1 cells undergoing apoptosis via exposure to 1 ┬ÁM of staurosporine.

Both macros are compatible with ImageJ.

Complete download (zip, 22.1 MiB)

Creator(s) Stephen Cross, Daniel Butt
Contributor(s) Paul Verkade, Alin Achim
Publication date 22 Nov 2023
Language eng
Publisher University of Bristol
Licence Non-Commercial Government Licence for public sector information
DOI 10.5523/bris.29u28g52tbulu2w9h0j5ewt1j1
Citation Stephen Cross, Daniel Butt (2023): Image analysis of apoptopic hallmarks. https://doi.org/10.5523/bris.29u28g52tbulu2w9h0j5ewt1j1
Total size 22.1 MiB

Data Resources